Reporter

Part:BBa_K2492000:Design

Designed by: ZHOU Xinyu   Group: iGEM17_SUSTech_Shenzhen   (2017-09-21)


GFP (optimised, for use in the C. elegans chassis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


Design Notes

As far as we considered, GFP can well expressed in bacteria,however, eukaryote like C.elegans may not have good result with GFP without intron. Adding introns may easily solve this problem.


Source

GFP optimized with three introns

References